Automated de novo protein sequencing of monoclonal antibodies
- 1 December 2008
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 26 (12) , 1336-1338
- https://doi.org/10.1038/nbt1208-1336
Abstract
De novo protein sequencing of monoclonal antibodies is required when the cDNA or the original cell line is not available, or when characterization of posttranslational modifications is needed to verify antibody integrity and effectiveness. We demonstrate that Comparative Shotgun Protein Sequencing (CSPS) based on tandem mass spectrometry can reduce the time required to sequence an antibody to 72 hours, a dramatic reduction as compared to the classical technique of Edman degradation. We therefore argue that CSPS has the potential to be a disruptive technology for all protein sequencing applications.Keywords
This publication has 19 references indexed in Scilit:
- Rapid cloning of high-affinity human monoclonal antibodies against influenza virusNature, 2008
- Dehydroalanine derived from cysteine is a common post‐translational modification in human serum albuminRapid Communications in Mass Spectrometry, 2008
- Shotgun Protein SequencingMolecular & Cellular Proteomics, 2007
- MSNovo: A Dynamic Programming Algorithm for de Novo Peptide Sequencing via Tandem Mass SpectrometryAnalytical Chemistry, 2007
- Protein identification by spectral networks analysisProceedings of the National Academy of Sciences, 2007
- Identification of post-translational modifications by blind search of mass spectraNature Biotechnology, 2005
- Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancerNature Reviews Drug Discovery, 2004
- Comparative genome assemblyBriefings in Bioinformatics, 2004
- PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometryRapid Communications in Mass Spectrometry, 2003
- Multiple sequence alignment with the Clustal series of programsNucleic Acids Research, 2003