Stable, Monomeric Variants of λ Cro Obtained by Insertion of a Designed β-Hairpin Sequence
- 21 December 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 250 (4988) , 1712-1715
- https://doi.org/10.1126/science.2148648
Abstract
Lambda Cro is a dimeric DNA binding protein. Random mutagenesis and a selection for Cro activity have been used to identify the contacts between Cro subunits that are crucial for maintenance of a stably folded structure. To obtain equivalent contacts in a monomeric system, a Cro variant was designed and constructed in which the antiparallel beta-ribbon that forms the dimer interface was replaced by a beta-hairpin. The engineered monomer has a folded structure similar to wild type, is significantly more stable than wild type, and exhibits novel half-operator binding activity.This publication has 14 references indexed in Scilit:
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