Restricted classes of immunoglobulin produced by a lymphoid cell line from a patient with agammaglobulinemia.

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Abstract
Restricted expression of immunoglobulin [Ig] by a long-term B [bone marrow-derived] lymphoid cell line derived from a patient with X-linked agammaglobulinemia was reported. The patient had peripheral blood B lymphocytes with surface IgD and IgM. Culture of the B cells in vitro with mitogens did not stimulate Ig secretion, although pokeweed mitogen stimulation resulted in the development of cytoplasmic IgD and IgM. The lymphoid cell line established from these B lymphocytes primarily bore surface IgD, with a small population of cells also bearing surface IgM. These cells also had cytoplasmic Ig, primarily IgD. The cell line did not have a cytoplasmic pool of Ig for export and did not secrete Ig. This B lymphoid cell line is an in vitro analogue of the arrest in differentiation of the patient''s B lymphocytes. The IgD-bearing phenotype may represent a normal transitional stage in the differentiation of B lymphocytes.