An automated procedure for measuring biotinidase activity in serum.
- 1 May 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 35 (5) , 831-833
- https://doi.org/10.1093/clinchem/35.5.831
Abstract
In this automated procedure for quantifying biotinidase activity in human serum, a manual colorimetric method that measures conversion of the enzyme's artificial substrate N-biotinyl p-aminobenzoate was modified for use with a Technicon AutoAnalyzer II. The intra-run replicate precision (CV) was 2.1% and the day-to-day CV was 4.6% for quality-control sera. Results were linearly related to biotinidase activity in serum over the complete range of clinically relevant values, 0.2 to 11.0 U/L. Moreover, results of the automated assay were not significantly different from those of the manual assay. Because the automated procedure is faster and more precise, we recommend it for population-based studies and some screening studies.This publication has 3 references indexed in Scilit:
- Biotinidase deficiency: the enzymatic defect in late-onset multiple carboxylase deficiencyClinica Chimica Acta; International Journal of Clinical Chemistry, 1983
- A Study of the Accuracy and Precision of Clinical Chemistry Determinations in 170 Canadian LaboratoriesClinical Chemistry, 1963
- REINIGUNG UND EIGENSCHAFTEN DER BIOTINIDASE AUS SCHWEINENIEREN UND LACTOBACILLUS CASEI1963