IMMUNOFLUORESCENT DEMONSTRATION OF BACTERIAL ANTIGEN IN EXPERIMENTAL PYELONEPHRITIS WITH ANTISERUM AGAINST COMMON ENTEROBACTERIAL ANTIGEN

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Abstract
Various strains of E. coli were used to produce experimental haematogenous pyelonephritis in rats. The persistence in the renal tissue of the common enterobacterial antigen (CA) shared by these micro‐organisms was then studied by means of indirect immunofluorescent technique with rabbit antiserum against CA. In fresh infections, CA could regularly be demonstrated in and around abscesses, in macrophages, in dilated tubules and in pelvic exudate. However, as early as 2 weeks in the course of the infection, CA was hardly detectable in macrophages. By means of specific antiserum against one of the strains (E. coli 04), it could, however, be shown that other antigens from this strain were still present in the macrophages in animals infected with this strain. In renal tissue with chronic inflammatory changes, examined more than 4 weeks after the bacterial injection, bacterial antigen could no longer be detected with antiserum against CA, whereas antiserum against E. coli 04 still revealed positive reactions on renal tissue from the rats infected with this strain.

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