Entry and survival ofSalmonella typhimuriumin dendritic cells and presentation of recombinant antigens do not require macrophage-specific virulence factors

Publisher Website
Google Scholar
Abstract
Macrophages have long been regarded as the main target encountered bySalmonella typhimurium,a Gram-negative facultative intracellular pathogen that invades the intestinal mucosa.S. typhimurium, however, are first internalized by dendritic cells. To gain new insights into the interactions betweenSalmonellaand the dendritic cells, we compared the fate of wild-typeS. typhimuriumand the virulence-attenuated PhoP constitutive (PhoPc) strain. The PhoPcstrain is impaired for entry and survival in mammalian cells and is poorly processed by macrophages for antigen presentation on MHC class II molecules. Here, we show that bone marrow-derived dendritic cells can similarly process and present a foreign antigen expressed by the invasive wild-type and the attenuated PhoPcS. typhimurium. This property correlates with equivalent entry and survival efficiencies of both strains in dendritic cells. In addition,Salmonellastrains mutated inmgtCB,sseC, andorfLgenes required for macrophage survival showed no defect in survival in dendritic cells. Together, these results indicate that uptake ofSalmonellaby dendritic cells and subsequent antigen processing and presentation do not depend on virulence factors important in macrophages.

This publication has 69 references indexed in Scilit: