On the mechanism of tRNATrp aminoacylation catalysed by beef tryptophanyl‐tRNA synthetase using presteady‐state kinetics
- 27 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 157 (1) , 210-214
- https://doi.org/10.1016/0014-5793(83)81147-8
Abstract
The dimeric tryptophanyl-tRNA synthetase from beef pancreas has been found to activate 2 tryptophans/mol enzyme [Eur. J. Biochem. (1982) 128, 389–398]. By using quenched-flow and stopped-flow methods under presteady-state conditions, we show that only one enzyme subunit operates at a time in the aminoacylation of tRNATrp and that the transfer reaction is not the rate-limiting step in the overall aminoacylation process.Keywords
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