Studies on the development of anti‐schistosomular surface antibodies by mice exposed to irradiated cercariae, adults and/or eggs of 5. mansoni
- 1 September 1983
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 5 (5) , 499-511
- https://doi.org/10.1111/j.1365-3024.1983.tb00764.x
Abstract
Summary Levels of antibody a binding to the schistosomulum surface and b mediating in vitro eosinophil‐dependent cytotoxicity of schistosomula were studied and compared to the in vivo levels of resistance to cercarial challenge in mice infected with irradiated cercariae, unirradiated cercariae of single or mixed sex, or injected with eggs. Antibody‐binding was assessed by counting the number of IgG‐Fc‐receptor bearing cells (P388D1 cells) adhering to mechanically‐transformed schistosomula. Significant levels of adherence occurred with sera taken from 1–2 weeks following exposure to irradiated cercariae, the level increasing gradually thereafter and being enhanced by repeated exposure. Sera from the bisexual infection showed a dramatic increase in binding activity between weeks 5–8, and with the single sex infections there was a steady rise up to week 10 followed by a sharp rise between weeks 10–12. Weekly injections of eggs produced a steady rise in serum binding activity. Sera taken just before challenge were also tested for their ability to mediate killing of schistosomula by eosinophil‐enriched preparations of heterologous rat peritoneal exudate cells in vitro. Significant levels of killing occurred with all sera, but the greatest lethal activity was found in sera from the egg‐injected, chronically‐infected and 200 male cercariae‐infected groups. This ranking did not correlate with in vivo resistance against challenge as assessed by worm recovery, the egg‐injected and single sex‐infected groups failing to manifest significant resistance.Keywords
This publication has 42 references indexed in Scilit:
- Passive immunization of mice againstSchistosoma mansoniwith an IgM monoclonal antibodyParasitology, 1982
- Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. Evidence that the mechanisms which mediate resistance during early patent infections may lack immunological specificityParasitology, 1982
- A new Fc receptor on mouse macrophages binding IgG3.The Journal of Experimental Medicine, 1981
- Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.The Journal of Experimental Medicine, 1977
- Acquisition of host antigens by youngSchistosoma mansoniin mice: correlation with failure to bind antibodyin vitroParasitology, 1975
- Acquired immunity toSchistosoma mansoniin the rat: measurement of immunity by the lung recovery techniqueParasitology, 1974
- Schistosoma mansoni: Changes in the outer membrane of the tegument during development from cercaria to adult wormInternational Journal for Parasitology, 1973
- The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitroInternational Journal for Parasitology, 1972
- Host Response to Eggs of Schistosoma Mansoni: III. The Role of Eggs in ResistanceThe Journal of Infectious Diseases, 1963