Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology
Open Access
- 1 January 2003
- journal article
- research article
- Published by Springer Nature in The Pharmacogenomics Journal
- Vol. 3 (1) , 41-52
- https://doi.org/10.1038/sj.tpj.6500147
Abstract
The present study successfully utilizes a new ADME Rat Expression Bioarray, containing 1040 metabolism- and toxicology-linked genes, to monitor gene expression from the livers of rats treated with carbon tetrachloride (CCl4). Histopathological analysis, hierarchical clustering methods, and gene expression profiling are compared between the control and CCl4-treated animals. A total of 44 transcripts were found to be altered in response to the hepatotoxin, 19 of which were upregulated and 25 were downregulated. Some of these gene expression changes were expected and concurred with previously published data while others were novel findings.Keywords
This publication has 82 references indexed in Scilit:
- Hepatic and intestinal cytochrome P450 3A activity in cirrhosis: Effects of transjugular intrahepatic portosystemic shuntsHepatology, 2001
- Unfolding of Microarray DataJournal of Computational Biology, 2001
- Systematic Analysis of DNA Microarray Data: Ordering and Interpreting Patterns of Gene ExpressionGenome Research, 2001
- Simultaneous Induction of Galectin-3 Phosphorylated on Tyrosine Residue, p21WAF1/Cip1/Sdi1, and the Proliferating Cell Nuclear Antigen at a Distinctive Period of Repair of Hepatocytes Injured by CCl4Biochemical and Biophysical Research Communications, 2001
- Differential alteration of cytochrome P450 isoenzymes in two experimental models of cirrhosisCanadian Journal of Physiology and Pharmacology, 2000
- Exploring the new world of the genome with DNA microarraysNature Genetics, 1999
- Options available — from start to finish — for obtaining expression data by microarrayNature Genetics, 1999
- From hybridization image to numerical values: a practical, high throughput quantification system for high density filter hybridizationsGenetic Analysis: Biomolecular Engineering, 1996
- Identification of Protein-Tyrosine Phosphatases Prevalent in Adipocytes by Molecular CloningBiochemical and Biophysical Research Communications, 1994
- Effects on experimental animals of long-term inhalation of trichloroethylene, carbon tetrachloride, 1,1,1-trichloroethane, dichlorodifluoromethane, and 1,1-dichloroethyleneToxicology and Applied Pharmacology, 1967