Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices
- 15 November 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 232 (1) , 211-215
- https://doi.org/10.1042/bj2320211
Abstract
Carbachol stimulation of muscarinic receptors in rat cortical slices prelabelled with myo-[2-3H]inositol caused the rapid formation of a novel inositol polyphosphate. Evidence derived from its chromatographic behaviour, and from the structure of the products formed in partial dephosphorylation experiments, suggests that it is probably D-myo-inositol 1,3,4,5-tetrakisphosphate. An enzyme in human red cell membranes specifically removes the 5-phosphate from it to form inositol 1,3,4-trisphosphate. It is suggested that inositol 1,3,4,5-tetrakisphosphate is likely to be a second messenger, and that it is the precursor of inositol 1,3,4-trisphosphate and possibly of inositol 1,4,5-trisphosphate.This publication has 25 references indexed in Scilit:
- The second messenger linking receptor activation to internal Ca release in liverNature, 1984
- Inositol Phospholipid Hydrolysis in Rat Cerebral Cortical Slices: I. Receptor CharacterisationJournal of Neurochemistry, 1984
- Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphateNature, 1983
- Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositolBiochemical Journal, 1983
- Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositidesBiochemical Journal, 1983
- The inositol trisphosphate phosphomonoesterase of the human erythrocyte membraneBiochemical Journal, 1982
- The stimulation of inositol lipid metabolism that accompanies calcium mobilization in stimulated cells: defined characteristics and unanswered questionsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1981
- Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipidsBiochemical Journal, 1981
- Isolation and separation of inositol phosphates from hydrolysates of rat tissuesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- On the nature of dialyzable phosphate associated with the di- and tri-phosphoinositides of brain phospholipidsBiochimica et Biophysica Acta, 1963