A new short chemical dehydration method for light microscopy preparations of plant material
- 1 May 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 54 (9) , 872-875
- https://doi.org/10.1139/b76-090
Abstract
Complete dehydration of plant material can be accomplished in one step for plastic-embedded samples by immersal in 2,2-dimethoxypropane (DMP). This chemical reacts with any water present to form methanol and acetone. It can be used in combination with a number of easily prepared fixatives, including 10% paraformaldehyde. paraformaldehyde–glutaraldehyde, and formalin – acetic acid – alcohol (FAA). The fixation image after use of DMP is not visibly different from that of other dehydrating procedures presently in common use.Keywords
This publication has 1 reference indexed in Scilit:
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