Purification and partial characterization of a DNA polymerase α species from calf thymus
Open Access
- 11 December 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (23) , 5703-5714
- https://doi.org/10.1093/nar/8.23.5703
Abstract
We have purified a DNA polymerase α species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly(dA)·(dt) 10.Keywords
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