Affinity chromatography of 3α-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Use of N,N-Dimethylformamide to prevent hydrophobic interactions between the enzyme and the ligand
- 31 May 1976
- journal article
- research article
- Published by Elsevier in Biochimica et Biophysica Acta (BBA) - Enzymology
- Vol. 438 (1) , 13-22
- https://doi.org/10.1016/0005-2744(76)90219-9
Abstract
No abstract availableThis publication has 27 references indexed in Scilit:
- ON THE 3α‐HYDROXYSTEROID DEHYDROGENASE FROM PSEUDOMONAS TESTOSTERONI. The effect of denaturing agentsInternational Journal of Peptide and Protein Research, 1975
- Biospecific Affinity Chromatography in Aqueous‐Organic Cosolvent MixturesEuropean Journal of Biochemistry, 1975
- 3alpha-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni: Kinetic Properties with NAD and Its Thionicotinamide AnalogueEuropean Journal of Biochemistry, 1975
- An enzymatic method for the determination of hormonal steroids in the urineJournal of Steroid Biochemistry, 1974
- Spacer arms in affinity chromatography: Use of hydrophilic arms to control or eliminate nonbiospecific adsorption effectsFEBS Letters, 1974
- Novel reagent for the fluorometric assay of primary aminesJournal of the American Chemical Society, 1972
- Affinity chromatography: Large‐scale purification of the soluble oestradiol‐17‐β dehydrogenase of human placentaFEBS Letters, 1972
- Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi‐substrate enzymesFEBS Letters, 1972
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- DISC ELECTROPHORESIS‐I BACKGROUND AND THEORY*Annals of the New York Academy of Sciences, 1964