MOLECULARLY CLONED AND EXPRESSED MURINE T-CELL GENE-PRODUCT IS BIOLOGICALLY SIMILAR TO INTERLEUKIN-3

  • 1 January 1985
    • journal article
    • research article
    • Vol. 13  (4) , 249-260
Abstract
Clonal lines of mouse inducer 1y1+1y2- inducer T-lymphocytes that depend for growth upon interleukin-2 were demonstrated to produce a factor that stimulates colony formation by bone marrow granulocyte-macrophage (GM-CFUc) progenitor cells and replication of factor-dependent mast cell/basophil and multipotential hematopoietic cell lines in vitro. The molecularly cloned and expressed gene product for this growth factor demonstrates the following activities in vitro: using fresh bone marrow or purified subpopulations of nonadherent cells from murine continuous bone marrow cultures as target cells: stimulation of colony formation by GM-CFUc, mast cell progenitor cells, multipotential granulocyte/erythroid/megakaryocyte/macrophage progenitor cells (CFU-GEMM) colonies, erythroid progenitor cells forming macroscopic bursts (BFUe), and megakaryocyte progenitor cells (CFU-mega). The gene product also supports growth of previously reported mast cell growth-factor-dependent cell lines and several classes of interleukin-3 (IL-3)-dependent hematopoietic progenitor cell lines that are multipotential (neutrophil/basophil/eosinophil or neutrophil/basophil/erythroid); or committed to granulocyte-macrophage, or mast cell/basophil differentiation. The gene product does not detectably support replication of IL-2-dependent murine T-cell lines. The biologic activity of the gene product was inhibited .gtoreq. 90% by rabbit antisera prepared against purified interleukin-3. This T-cell derived lymphokine gene product is biologically very similar to interleukin-3.

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