Structure of an antibody combining site by magnetic resonance

Abstract
The structure of the combining site of the DNP [dinitrophenol] binding Ig[immunoglobulin]A mouse myeloma protein MOPC 315 was determined by a combination of high resolution NMR, ESR, model building and chemical modifications. This approach yields the general dimensions of the site, its polarity and asymmetry features, the assignment of the DNP-contact residues and their 3-dimensional coordinates.