Sensitivity of Normal and Leukemic Lymphocytes and Leukemic Myeloblasts to Heat2

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Abstract
Purified suspensions of lymphocytes were prepared from blood of normal persons and from patients with chronic lymphocytic leukemia. The suspensions were exposed to 42–50 C and then incubated at 37 C. The number of viable lymphocytes was counted on the 7th day; viability was judged by morphologic integrity of the cells as seen with a phase-contrast microscope. The leukemic lymphocytes were, in general, more sensitive to exposure to 43–50 C than normal lymphocytes. For example, exposure to 43 C for 2 hours produced a 40–100% cytocidal effect on lymphocytes from 16 of 19 leukemic patients and produced less than 40% effect on lymphocytes from all 18 normal persons tested. Exposure to 42 C produced approximately the same effect on normal and leukemic lymphocytes. These and other data suggested that 43 C was a critical temperature for the viability of normal and leukemic lymphocytes. Similar tests were done on myeloblasts from blood of 4 patients with acute granulocytic leukemia. The myeloblasts of only 1 patient were as sensitive to heat as leukemic lymphocytes.

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