Determination of 7-Hydroxymethotrexate in Human Plasma by Reversed Phase High-Performance Liquid Chromatography
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Oncology
- Vol. 43 (2) , 86-88
- https://doi.org/10.1159/000226340
Abstract
A rapid and sensitive reversed phase HPLC method was developed for the detection of 7-hydroxymethotrexate (7-OHMTX) which is the main metabolite in human plasma after high-dose methotrexate (MTX) therapy. The simple clean-up procedure of plasma samples described allows the measurement of 7-OHMTX during clinical management of MTX infusions. Elimination kinetics of 7-OHMTX are given from 53 drug cycles of 6 patients.Keywords
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