THE COLCHICINE TEST AS A METHOD FOR DETERMINING THE TIME OF ONSET AND THE DURATION OF ACTION OF MALE ACTIVE SUBSTANCES

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Abstract
Modification of the test of Loewe and Voss by the use of colchicine permits of a finer analysis of the effect of [male] hormones on the seminal vesicles than by the usual methods in which the enlargement of the whole seminal vesicle is observed. Observation of the action or a single inj. of a [male] active substance throughout its course of action is made possible. On comparison of different active substances the more accurate histological analysis probably permits the establishment of differences in the mechanism of the action. The first profuse formation of mitoses in the seminal vesicles of young castrated mice is observed 24 to 25 hrs. after inj. of 150 [gamma] of testosterone and 27 to 31 hrs. after inj. of 180 [gamma] of testosterone propionate. Their activity is still maintained after 58 hrs., while doses 10 times smaller develop much weaker activity after this time. Testosterone propionate shows the most numerous mitoses and the most uniform distr. The addition of "X" substance to testosterone increases the activity of the latter.

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