Disc Flotation Test for Measurement of Catalase Activity in Milk

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Abstract
The method consists of absorbing milk on a 0.5-inch disc of highly absorbent paper and dropping the disc into a 16 by 125 mm test tube containing 5 ml of 3% H2O2. The time required for the disc to float to the surface is recorded. Statistical analysis of leucocyte counts made on milk by the direct microscopic method, and catalase activities by the disc flotation method gave a correlation coefficient of -0.75. Individual quarter samples of milk were examined to determine the relationship between leucocyte count, disc flotation time and California mastitis test reaction. Leucocyte counts and disc flotation times were in better agreement than leucocyte counts and California mastitis test reactions. The disc flotation test is simple, rapid, accurate and requires very little equipment. It can detect catalase activity in bulk milk, milk from individual cows or individual quarter samples.

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