Duck Hepatitis Virus in Duck Embryo Liver Cell Cultures

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Abstract
A field strain of duck hepatitis virus was carried 20 serial passages in duck embryo liver cell cultures. Varying degrees of fragmentation of the cells and cytolysis were observed in the virus-inoculated cell cultures from the 4th to the 20th passages, but the changes were mild and inconspicuous. Pyknotic nuclei and cytolytic cellular debris were prominent in the virus-inoculated and stained cell cultures. The titer of the cell culture virus in ducklings decreased with continued passages, and the virus was not detected at the 4th passage. Its titer in chicken embryos remained at approximately 101.7 ID50/0.1 throughout the 20 passages. The cell culture virus was identified by neutralization tests in chicken embryos and resistance tests in ducklings as well as by production of specific lesions in chicken embryos. The duck embryo liver cell culture system was not considered to be suitable for virus propagation.

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