Synergistic antiproliferative effects of glucocorticoids and interferon-? on some lymphoid cell lines

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Abstract
The Daudi B lymphoblastoid cell line was previously demonstrated to be highly sensitive to the antiproliferative effect of recombinant interferon-αA (rlFN-α). In the present study, glucocorticoid hormones were shown to act synergistically with rlFN-α to further increase the sensitivity of Daudi cells to rlFN-α.At 106M, dexamethasone, prednisolone, or hydrocortisone alone had little effect on Daudi cell growth, but they greatly potentiated the antiproliferative activity of rlFN-α. The synergy between rlFN-α and glucocorti-coids on Daudi cells was not related to the inhibitory effects of glucocorticoids on prostaglandin or leukotriene synthesis, since no synergy was observed between rlFN-α and indomethacin or nordihydroguaiaretic acid. Glucocorticoids and rlFN-α also had appreciable synergistic antiproliferative effects on two out of five other IFN-sensitive lymphoid cell lines. When Raji B lymphoblastoid cells, which were quite resistant to the antiproliferative effect of rlFN-α, were treated with the combination of glucocorticoids and rlFN-α, no significant synergistic effects were observed. The synergistic antiproliferative effects of glucocorticoids and rlFN-α observed with some IFN-sensitive lymphoid cell lines in this in vitro study may have clinical relevance in the treatment of certain lymphoid malignancies that are sensitive to rlFN-α therapy.
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