Protein kinase C(19–31) pseudosubstrate inhibition of insulin action in rat adipocytes

Abstract
Treatment of intact adipocytes with the autoregulatory PKC pseudosubstrate PKC(19–31) inhibited insulin‐stimulated hexose uptake and lipogenesis, with no effect on basal values. The effect was dose‐dependent with respective IC10 values of 30 μM and 600 μM for insulin‐stimulated hexose uptake in electroporated and intact adipocytes. These studies indicate that PKC may play a role in the mediation of insulin action in adipocytes.