The involvement of photosynthesis in inducing bud formation on excised leaf segments of Heloniopsis orientalis (Liliaceae)

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Abstract
The role of photosynthesis in inducing adventitious bud formation on leaf segments of Heloniopsis orientalis was investigated. The effect of white light reached a maximum at about l25 J·m−2·sec−1. White, red, blue and far-red light were effective in inducing bud formation, but green light was not. In darkness, bud formation was induced if sugar was added to the nutrient medium. The photosynthetic inhibitors DCMU and AT blocked the effect of light. Bud formation was inhibited in CO2-free air. The requirement of sucrose for bud formation in darkness could be replaced by citrate. It was concluded from these results that light appears to induce buds on leaf segments through some processes dependent upon photosynthesis.