On‐line liquid chromatography/thermospray mass spectrometry in the analysis of oligosaccharides
- 1 March 1992
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 6 (3) , 197-202
- https://doi.org/10.1002/rcm.1290060309
Abstract
The analysis of intact neutral oligosaccharides by on-line liquid chromatography/thermospray mass spectrometry is described. Molecular-weight information on oligomers up to a degree of polymerization of 10 is obtained using an aqueous mobile phase containing 10−4 mol/L sodium acetate, which was found to be compatible with thermospray interfacing and ionization. Ions due to sodiated and disodiated oligosaccharides are observed under these conditions without fragmentation. The aqueous 10−4 mol/L sodium acetate mobile phase is demonstated to be applicable in the separation of mixtures of oligosaccharides on a reversed-phase octadecyl-modified silica column.Keywords
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