Screening and Application of Microbial Esterases for the Enantioselective Synthesis of Chiral Glycerol Derivatives
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 3 (1-2) , 137-144
- https://doi.org/10.3109/10242429008992056
Abstract
A number of enzymes with reasonable to excellent selectivities for the conversion of prochiral 2-benzylglyceroldiacetate 1c into optically active monoacetate 2c could be identified in a screening of microbial esterases and lipases. For the synthesis of the S-enantiomer of 2c by hydrolysis, two enzymes (from Aspergillus fumigatus and from Mucor javanicus) were identified which give the product with an e.e.-value of 75%. The R-enantiomer can be obtained with a purified lipase/esterase from Pseudomonas fluorescens in 96% e.e. The optical purity of the product was determined without derivatisation by chromatography on a chiral HPLC-column.Keywords
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