EXAMINATION OF ENZYME-ALTERED FOCI WITH GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE, ALDEHYDE DEHYDROGENASE, GLUCOSE-6-PHOSPHATE-DEHYDROGENASE, AND OTHER MARKERS IN METHACRYLATE-EMBEDDED LIVER

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  • 1 January 1987
    • journal article
    • research article
    • Vol. 56  (1) , 96-100
Abstract
F344 Male rats weighing between 90 and 110 gm were given 90 ppm diethylnitrosamine in their drinking water for 5 weeks. Seven weeks after the administration of carcinogen was completed, the rats were sacrificed and sections of their livers were embedded in methacrylate. Serial sections 2 or 4 .mu.m in thickness demonstrated the presence of .gamma.-glutamyl transpeptidase, acid phosphatase, adenosine triphosphatase, aldehyde dehydrogenase, alkaline phosphatase, .alpha.-naphthyl butyrate esterase, DT diaphorase, glucose-6-phosphate dehydrogenase, and 5''-nucleotidase activity and glycogen. The use of 4-.mu.m sections of methacrylate-embedded tissue allows the evaluation of many more phenotypic markers in serial sections than is currently possible with frozen sections.