Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation‐HPLC‐MS
Open Access
- 3 July 1998
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 430 (3) , 419-423
- https://doi.org/10.1016/s0014-5793(98)00706-6
Abstract
A quantitative method for detection of amyloid β peptides using immunoprecipitation‐HPLC‐mass spectrometry (IP‐LC‐MS) is described. Comparison of IP‐LC‐MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates. The use of IP‐LC‐MS not only allows a quantitative method for Aβ 1–40 and Aβ 1–42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.Keywords
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