Transcription of ribosomal genes can cause nondisjunction
Open Access
- 12 June 2006
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 173 (6) , 893-903
- https://doi.org/10.1083/jcb.200511129
Abstract
Mitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These findings demonstrate that highly transcribed loci can cause chromosome nondisjunction.Keywords
This publication has 46 references indexed in Scilit:
- Condensin Loaded onto the Replication Fork Barrier Site in the rRNA Gene Repeats during S Phase in a FOB1-Dependent Fashion To Prevent Contraction of a Long Repetitive Array in Saccharomyces cerevisiaeMolecular and Cellular Biology, 2006
- Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication forkGenes & Development, 2005
- Impairment of replication fork progression mediates RNA polII transcription-associated recombinationThe EMBO Journal, 2005
- Pre-18S Ribosomal RNA Is Structurally Compacted into the SSU Processome Prior to Being Cleaved from Nascent Transcripts in Saccharomyces cerevisiaeMolecular Cell, 2004
- Cdc14 Phosphatase Induces rDNA Condensation and Resolves Cohesin-Independent Cohesion during Budding Yeast AnaphaseCell, 2004
- Mitotic Exit Network Controls the Localization of Cdc14 to the Spindle Pole Body in Saccharomyces cerevisiaeCurrent Biology, 2002
- Regulation of the Bfa1p–Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14pThe Journal of cell biology, 2002
- DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphataseMolecular Genetics and Genomics, 1998
- A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activitiesGenes to Cells, 1996
- Segregation of the nucleolus during mitosis in budding and fission yeastCell Motility, 1991