Diffusibility of Dyes in Buffered and Unbuffered Solutions.

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Abstract
The diffusion of 13 basic dyes through a cellophane membrane was studied in acid-buffered and in unbuffered solns. The rate of diffusion depends not only on the concn. gradient but also the medium. Diffusion into a concd. buffer is faster than into a diluted one. The concd. buffer represses the ionization, and thus retards back diffusion. If the buffer compartment is diluted in the same proportion as the dye compartment, the rate of diffusion of the dyes is only slightly affected, if at all, by a 4-fold dilution. With methylene blue the membrane has, in different media, a different diffusion capacity. This work suggests that conclusions based on diffusion measurements made on simple aqueous solns. may not be equally valid for biological systems.
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