Single crystals of bacteriophage T7 RNA polymerase
- 1 January 1989
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 5 (4) , 266-270
- https://doi.org/10.1002/prot.340050403
Abstract
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self-rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.Keywords
This publication has 17 references indexed in Scilit:
- Replication of RNA by the DNA-dependent RNA polymerase of phage T7Cell, 1989
- REVIEWBiological Chemistry Hoppe-Seyler, 1988
- The use of an imaging proportional counter in macromolecular crystallographyJournal of Applied Crystallography, 1987
- T7 lysozyme inhibits transcription by T7 RNA polymeraseCell, 1987
- Cloning and expression of the gene for bacteriophage T7 RNA polymerase.Proceedings of the National Academy of Sciences, 1984
- Nucleotide sequence of the gene for bacteriophage T7 RNA polymeraseJournal of Molecular Biology, 1984
- Mechanisms for the Initiation of Bacteriophage T7 DNA ReplicationCold Spring Harbor Symposia on Quantitative Biology, 1983
- Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatographyBiochemistry, 1975
- Structure of a dimeric fragment related to the lambda-type Bence-Jones protein: A preliminary studyJournal of Molecular Biology, 1974
- New RNA Polymerase from Escherichia coli infected with Bacteriophage T7Nature, 1970