Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon.

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Abstract
The promoter region of the rrnB rRNA gene of E. coli was ligated within the epimerase gene (galE) of the galactose operon in a .lambda. phage vector. The recombinant .lambda. phage was characterized by restriction mapping and assays of both galK (galactokinase) gene activity and galactose mRNA hybridization. In such lysogens, expression of the fused galactose operon occurs as a function of growth rate in a manner characteristic of rRNA gene expression and is subject to stringent control by amino acid availability for protein synthesis. Galactose mRNA arising from the ribosomal promoter is not as metabolically stable as rRNA.