An Appraisal of an Autoradiographic Technique for Enumeration of Antibody Containing Cells in Response to Salmonella Somatic Polysaccharide

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Abstract
Using biosynthetically labeled C14 Sal- monella enteritidis somatic polysaccharide, optimum conditions for the use of an autoradiographic technique to enumerate and describe antibody containing cells were determined. With this technique the immume response in mice and rabbits to a single injection of unlabeled S. enteritidis, somatic polysaccharide was determined and the data compared to that obtained by localized homolysis in gel and passive hemagglutina-tion. At day 10 in the endotoxin stimulated rabbit node preparations the response was one of marked cellular proliferation. Labeled anti -gen was bound principally to lymphoblasts and large and medium sized lymphocytes, but some plasmoblasts and proplasmacytes were labeled also. Only a rare small lymphocyte or plasma cell was labeled. Label was also observed over eosinophils and reticular cells. The presence of antibody in the cells in the lymphocyte and plasma cell series results from antibody synthesis, while its presence in eosinophils represents the adherence of cytophilic antibody. The autoradiographic technique has 2 advantages which the techniques of localized gel hemolysis and passive hemagglutination lack: it allows for the simultaneous enumeration and morphologic evaluation of antibody containing cells and is sensitive to their total current of antibody.

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