Trans-Kingdom Conjugation Between Agrobacterium tumfaciens and Saccharomyces cerevisiae, a Bacterium and a Yeast
Open Access
- 1 January 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 37 (1) , 103-106
- https://doi.org/10.1093/oxfordjournals.pcp.a028906
Abstract
For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.Keywords
This publication has 0 references indexed in Scilit: