Effect of Disodium Phosphonoacetate and Iododeoxyuridine on the Multiplication of African Swine Fever Virus in vitro

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Abstract
Disodium phosphonoacetate (PAA) inhibits the replication of African swine fever virus (ASFV). The action of this compound was compared with the inhibitory capacity of iododeoxyuridine (IDU) upon ASFV growing in [African green monkey kidney] Vero cells. The study was done by the immunofluorescence technique in order to detect formations of cytoplasmic virus antigens and inclusion bodies; both were inhibited by IDU and PAA. At 100 .mu.g/ml, IDU blocked completely the multiplication of ASFV and with PAA, a few scattered cells showed positive fluorescence. The infectivity of the virus was reduced 1-5 log depending upon drug concentrations and time of exposure to the drugs. Inhibition of ASFV replication by PAA suggests that this virus, like herpesviruses, involves a virus-specific DNA polymerase in its replication mechanism.