Effect of chromosomal position on amplification of transfected genes in animal cells

Open Access

1 February 1984

journal article
research article
Published by Springer Nature in Nature

Vol. 307 (5951) , 516-520
https://doi.org/10.1038/307516a0

Abstract

A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.

This publication has 46 references indexed in Scilit:

DOUBLE MINUTES AND HOMOGENEOUSLY STAINING REGIONS: Gene Amplification in Mammalian Cells
Annual Review of Genetics, 1982
Single-copy and amplified CAD genes in Syrian hamster chromosomes localized by a highly sensitive method for in situ hybridization.
Molecular and Cellular Biology, 1982
Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.
Proceedings of the National Academy of Sciences, 1982
Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the dihydrofolate reductase gene.
Proceedings of the National Academy of Sciences, 1981
Cloning of DNA from double minutes of Y1 mouse adrenocortical tumor cells: Evidence for gene amplification
Cell, 1981
Amplification and rearrangement of integrated SV40 DNA sequences accompany the selection of anchorage-independent transformed mouse cells
Cell, 1980
Specifically altered metaphase chromosomes in antifolate-resistant Chinese hamster cells that overproduce dihydrofolate reductase
Cancer Genetics and Cytogenetics, 1980
Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells
Cell, 1980
Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells.
The Journal of cell biology, 1979
Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.
Proceedings of the National Academy of Sciences, 1978