EFFECT OF SMALL DOSES OF ACTINOMYCIN D ON OESTROGEN-INDUCED UTERINE CHANGES IN THE IMMATURE RAT

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Abstract
Actinomycin D has been intensively studied by a number of investigators interested in the inhibition of hormonal activity. These studies were recently reviewed by Samuels (1964) and Litwack & Kritchevsky (1964). It is generally agreed that this antibiotic acts by the inhibition of RNA synthesis and, more specifically, blockage of DNA-directed RNA synthesis. The doses of actinomycin D employed have generally been large enough to have toxic effects in addition to antagonizing hormonal action specifically. The effects of relatively low doses of actinomycin D upon oestrogen-induced uterine growth were examined in the present study. Oestradiol benzoate, actinomycin D or both compounds in sesame oil were administered s.c. once daily for 4 consecutive days to dose groups of 20–35 immature (body weight 40–45 g.) female Sprague-Dawley rats obtained by caesarean section. On the 5th day the animals were killed, the uteri removed, weighed and quick-frozen. They were kept frozen until assayed

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