Purification and Resolution of the Optical Isomers of Labeled Tryptophan Metabolites on Powdered Paper Columns
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 4 (1) , 47-64
- https://doi.org/10.1080/00327487408068185
Abstract
Procedures are presented illustrating methods of preparing glass columns (3.5 to 9.9 cm in diameter and 117 to 170 cm in height) packed with powdered paper for chromatography. A reaction mixture of anthranilic acid-l-C14, 3-hydroxyanthranilic acid-l-C14 and 5-hydroxyanthranilic acid-1-C14 was placed on a powdered paper column and the components separated by passing different organic solvent systems through the column. DL-Kynurenine-keto-C14, DL-Kynurenine-ring-2-C14, Hydroxy-DL-Kynurenine-keto-C14, and DL-Tryptophan-7a-C14 were resolved into the pure D- and L-isomers by the use of the powdered paper columns with very little loss of material.Keywords
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