Mechanical Transmission of a Plant Tumor Virus to an Insect Vector

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Abstract
Non-viruliferous leafhoppers, Agallia constricta, were caged for 4 wks. at 25[degree]C on crimson clover plants (Trifolium incarnatum) affected with wound tumor disease caused by the virus Aureogenus magnivena. They were then collected, ground at 0[degree]C with an equal wt. of 0.25 [image] NaCl, the brei centrifuged at 3500 RPM for 5 min. and the supernatant injd. into the abdomens of 28 virus-free nymphs. Of the 16 leafhoppers that survived, 12 proved to be infective when caged on disease-free clover plants. Similar expts. using diluted supernatant showed that some insects became infective when injd. with dilutions as high as 10-3. There was a long incubation period. No accidental infection of clover was observed in these expts. or during 2 previous yrs.
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