Measurement of Testosterone Excretion and Production Rate by Glass Paper Chromatography1

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Abstract
A recently described method for separation of urinary 17-KS by glass paper chromatography has been modified to include a reliable and specific technique for the isolation and measurement of testosterone in human urine. Testosterone production rates are determined by the isotope dilution principle with intravenous injection of testosterone 1,2-H3 and isolation of tritiated testosterone from urine. Urinary testosterone is quantitated by a fluorimetric method with a sensitivity of 0.01 [mu]g. Values for testosterone excretion and production rates in normal and abnormal subjects are presented. Because of its simplicity, rapidity and precision, this glass paper chromatographic method for measurement of testosterone and the individual urinary 11-desoxy-17-KS should provide a useful clinical technique for studies of androgen metabolism in man.