TISSUE-CULTURE OF LENS EPITHELIAL-CELLS FROM NORMAL AND NAKANO MICE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 16  (3) , 243-246
Abstract
Lens epithelial cells from normal and Nakano adult mice were cultured for over 1 yr, and the cells retained certain differentiated characteristics. Fluorescent antibody to mouse .gamma. crystallin reacted with the spherical lentoid bodies which appeared approximately 2 wk after the start of the culture. The lentoid bodies also contained cells which had few cell organelles and homogeneous cytoplasms. Both .gamma. crystallin production and loss of cellular organelles were characteristics of differentiated fiber cells rather than epithelial cells. An inhibitor of the Na+-K+ ATPase was responsible for the hydration and subsequent cataract formation in the Nakano mouse. The inhibitor of the Na+-K+ ATPase was demonstrated in the lens cells in culture from the Nakano mice, but no inhibitory activity was detected in the cultures from normal mice.

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