Murine Glial Cells Regenerate NAD, After Peroxide‐Induced Depletion, Using Either Nicotinic Acid, Nicotinamide, or Quinolinic Acid as Substrates
Open Access
- 1 April 1998
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 70 (4) , 1759-1763
- https://doi.org/10.1046/j.1471-4159.1998.70041759.x
Abstract
The potential for regeneration of intracellular pyridine nucleotide levels from different precursors, after peroxide‐induced NAD depletion, in cultured glial cells was investigated. Cultured murine glial cells showed a decrease in intracellular NAD levels of >40% after treatment with H2O2 (100 µM). Removal of the H2O2 followed by a 2‐h incubation did not result in NAD recovery in the absence of precursors. However, NAD levels increased significantly in these cells after the following substrate additions, at minimum effective concentrations of 1 mM for quinolinic acid (QUIN), 500 µM for nicotinamide, and 2 µM for nicotinic acid. The regeneration of significant amounts of NAD from nicotinic acid at doses 250 and 500 times lower than either nicotinamide or QUIN indicates a preferred route for NAD biosynthesis in glial cells in vitro, probably via nicotinic acid phosphoribosylation.Keywords
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