A comparison of cAMP phosphodiesterases in normal, malignant, and somatic cell hybrids

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Abstract
Hybrids (PCM) between a malignant mouse lymphoma suspension cell line (P388F-36) and a normal Chinese hamster fibroblastic cell line (Ch23) have already been isolated in this laboratory. Investigations were carried out on the cAMP phosphodiesterases of the parents and two of these hybrids—PCM2 and PCM3. PCM3 shows a rather unusual growth characteristic in that a considerable proportion of the cells exist at any one time either in suspension or only loosely attached to the substratum, the remaining cell population existing in a monolayer form. It was found that each cell line exhibited multiple forms of the enzyme with varying affinities for cAMP. Both parents, although different, contained high-, low-, and extra-high apparent K m forms of the enzyme. The hybrids exhibited characteristics of both parental systems but were different from each other. Neither hybrid exhibited a high-K m enzyme, but both exhibited two low-K m forms. There was also a slight variation between monolayer and suspension cells of PCM3 hybrid. An attempt has been made to explain these phenomena with respect to hybridization and the growth characteristics of the cells.

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