Induction of β-D-Glucosidase inStreptomyces granaticolor

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Abstract
β-d-Glucosidase inStreptomyces granaticolor is an inducible enzyme. Methyl-β-d-glucoside or cellobiose, added to a glycerol-containing medium, are most suitable inducers. The activity of β-d-glucosidase in a culture fully induced by cellobiose is 50 times higher than the basal level of the enzyme. β-d-Glucosidase is an intracellular enzyme, whose inducibility differ with culture age and reaches its maximum in a 10-h-old mycelium. The enzyme synthesis begins 2 h after the addition of the induced and reaches its maximum after a 10-h-induction.