Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

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Abstract
Cultured rat spermatogonia, genetically modified via a transposon-based gene trap, are inserted into the testes of sterile rats and give rise to mutant progeny with many different genotypes. Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.