Histogenesis of Newt Blastema

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Abstract
Radiothymidine labeling of connective tissue in the amputation stump was limited to the periosteum, perichondrium, Schwanncells, perivascular interstitial cells and the early blastema. The blastema site showed a few labeled cells as soon as 72 hours and continued to form a small aggregate of cells by 6 days. The labeled Schwann cells and perivascular interstitial cells between muscle fibers appear to contribute to the initiation of the early blastema. No mature cells were labeled. Radiothymidine injection at 6 days following amputation labels the early blastema aggregate. The number of labeled cells and labeled mitotic figures increased between 6 and 16 days. The increase labeled cell number is a function of mitotic activity. It was concluded that the blastema cells arise from the perivascular interstitial cells and Schwann cells.