Fusarium avenaceum (Corda ex Fr.) Sacc. has been shown for the first time to be a seed-borne pathogen of subterranean clover (T. subterraneum L.). In laboratory tests symptoms of subterranean clover root rot occurred on plants grown from infected seed. F. oxysporum (Schlecht.) also was seed-borne. It was only weakly pathogenic, although isolated more frequently from diseased roots than F. avenaceum. Pythium sp. was not detected on seed and rarely isolated from roots of diseased field plants, but it was highly pathogenic to subterranean clover roots in laboratory tests. F. avenaceum was detected in 81 out of 85 seed lines tested, which comprised 11 cultivars, from four Australian States. Infection with F. avenaceum occurred on up to 27% of the seed in a sample. An application of benomyl (0.63 g/kg seed) controlled seed infection.