Effect of NAA on Shoot Production in Vitro from BA-pretreated Petunia Leaf Explants 1

Abstract
Leaf explants from ‘Sugar Daddy’ and ‘Sugar Plum’ petunia (Petunia hybrida L.) were pretreated in solutions of 0, 200, 400, and 800 mg/liter 6-benzylamino purine (BA) and were placed in a cytokinin-free modified Murashige and Skoog (MS) medium, to which 0.05, 0.1, or 0.2 mg/liter naphthaleneacetic acid (NAA) were incorporated to test interaction. NAA at 0.05 or 0.1 mg/liter increased shoot number, fresh weight, and shoot quality rating for ‘Sugar Daddy’, while in ‘Sugar Plum’ addition of NAA increased shoot fresh weight and improved shoot quality rating without any effect on shoot number.

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