INTERSTITIAL CELL STIMULATING HORMONE. II. METHOD OF PREPARATION AND SOME PHYSICO-CHEMICAL STUDIES11

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Abstract
The prep, of a highly purified globulin fraction, from sheep pituitary extracts, having 2 biological effects (interstitial cell repairing and antagonistic) is described. The protein contains 4.45% hexose. The use of the orcinol reaction indicates that the hexose is not galactose or glucose; it is probably mannose. The prep, also contains glucosamine; the molar ratio of mannose to glucosamine is approximately 11. Judged by ketone treatment free ammo groups appear essential for both interstitial cell stimulating and "antagonistic" properties of the protein. Electrophoresis expts. also indicate that the prep, is a single substance. There is no evidence from these studies that the "antagonism" shown by these preps, is caused by a substance separate from that causing interstitial cell repair.