Use of monoclonal antibodies for detection of infectious pancreatic necrosis virus by the enzyme-linked immunosorbent assay (ELISA)

1 January 1990

journal article
research article
Published by Inter-Research Science Center in Diseases of Aquatic Organisms

Vol. 8 (3) , 157-163
https://doi.org/10.3354/dao008157

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for identification of the Sp strain of infectious pancreatic necrosis virus (IPNV). This assay employed 2 monoclonal antibodies directed against 2 non-overlapping epitopes of the minor structural viral protein. It was used to demonstrated the virus antigen in cell cultures and was able to detect 10 ng ml-1 of purified virus or 104 TCID50 ml-1 in tissue culture fluid. The assay could be reduced to one step and be performed within 90 min with good sensitivity. No antigenic variation, affecting the epitopes recognized by these monoclonal antibodies, was observed on 17 IPNV isolates of Sp serotype tested.

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