Ca2+ binding of latent transforming growth factor‐β1 binding protein
- 5 April 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 320 (2) , 140-144
- https://doi.org/10.1016/0014-5793(93)80079-a
Abstract
Latent transforming growth factor-β1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-β1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-β1 complex. The binding of Ca2+ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-β1 is not influenced by the presence of Ca2+. The possible significance of Ca2+ binding of LTBP is discussed.Keywords
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